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PZA and INH treatment to the in vitro granulomas generated from T2DM subjects at 3-months post- L-GSH treatment also resulted in a significant 3-fold decrease in the levels of IL-10 when compared the untreated (control) category before L-GSH supplementation ( Figure 7B )

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The cells were cultured in Eagles minimum essential medium (ThermoFisher Scientific) or DMEM F-12 (ThermoFisher Scientific) respectively, supplemented with 10% Fetal Bovine Serum (Gibco) and 1% penicillinstreptomycin (ThermoFisher Scientific), in a humidified incubator maintaining 5% CO 2 at 37 C